Chlorophyll chromatographic separation techniques

The use of appropriate analytical standards is important for successful chromatographic separation, identification, and quantification of chlorophyll derivatives. While chlorophyll a and b derivatives are readily available commercially (Sigma-Aldrich) both metal-free pheophytins and metalloporphyrin analogs such as Cu2+ and Zn2+ pheophytins are not. In most instances, these derivatives must be prepared from the parent Mg-chlorophyl standards prior to use. These simple synthesis techniques are based on the work of Schwartz (1984) and are to be utilized for the rapid and efficient preparation of metal-free, Cu2+ and Zn2+ pheophytin derivatives in quantities appropriate only for analytical implementation. 

Tests carried out with the high performance liquid chromatographic technique endorsed the claim that it causes negligible degradation of both the chlorophylls and the xanthophylls. A more efficient separation of... 

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