Chlorophyl monomolecular layers

Optical reflection from a monomolecular film must be measured from the interface with a very small amount of material present. Therefore, in these methods, repeated interaction of the light beam with one or more identical films is generally employed. The simplest way to observe a light beam that has passed through several identical monolayers is to transfer portions of the layer to suitable transfer end plates, which are then stacked and examined in a conventional spectrophotometer. This method was used where monolayers of chlorophyll were deposited on glass slides by the LB method. Ferrodoxin and chlorophyll monolayers were investigated by measuring the spectra (550-750 nm) of these films at the interface. 

---