Ovary cells, Chinese hamster

L Na -iiidepeiideiit Braiiched-chaiii and aromatic amino acids Ehrlich ascites cells Chinese hamster ovary cells Hepatocytes... 

FIGURE 2.11 Receptor-occupancy curves for activation of human calcitonin type 2 receptors by the agonist human calcitonin. Ordinates (response as a fraction of the maximal response to human calcitonin). Abscissae (fractional receptor occupancy by human calcitonin). Curves shown for receptors transfected into three cell types human embryonic kidney cells (HEK), Chinese hamster ovary cells (CHO), and Xenopus laevis melanophores. It can be seen that the different cell types lead to differing amplification factors for the conversion from agonist receptor occupancy to tissue response. 

Dornase alfa is produced by genetically engineered Chinese Hamster ovary cells containing DNA encoding for the native human protein deoxyribunuclease I. It is purified by tangential flow filtration and column chromatography. 

Results of methyl parathion assays involving effects on chromosomes have also been contradictory. For sister chromatid exchange, Waters et al. (1982) reported a positive response in Chinese hamster ovary cells only in the presence of metabolic activation system, while methyl parathion tested positive without a metabolic activation system in Chinese hamster V79 cells (Chen et al. 1981), cultured normal human lymphoid cells (Chen et al. 1981 Gomez-Arroyo et al. 1987 Sobti et al. 1982), and Burkitt s l5miphoma cells (Chen et al. 1981). Chen et al. (1981) found a significant dose-related increase in sister chromatid exchange in both hamster and human cultured cells, but dose-related cell cycle delays were less pronounced in human cell lines than in V79 cells. Negative results were obtained for chromosomal aberrations in human lymphocytes without a metabolic activation system (Kumar et al. 1993). 

Belcourt, M.F. Hodnick, W. F. Rockwell, S. Sartorelli, A. C. Bioactivation of mitomycin antibiotics by aerobic and hypoxic Chinese hamster ovary cells overexpressing DT-diaphorase. Biochem. Pharm. 1996, 51, 1669-1678. 

Shyamala V, Khoja H. Interleukin-8 receptors R1 and R2 activate mitogen-activated protein kinases and induce c-fos, independent of Ras and Raf-1 in Chinese hamster ovary cells. Biochemistry 1998 37(45) 15918-15924. 

J Riordan, V Ling. (1979). Purification of P-glycoprotein from plasma membrane vesicles of Chinese hamster ovary cell mutants with reduced colchicine permeability. J Biol Chem 254 12701-12705. 

Chinese hamster ovary cells Syrian hamster embryo cells Chromosomal aberration, DNA repair, mitotic disturbance NA + Bauchingerand Schmid 1972 Costa et al. 1982 Robison et al. 1984 Zelikoff et al. 1988 Ariza et al. 1998... 

Leurs, R., Traiffort, E., Arrang, J. M. et al. (1994). Guinea pig histamine HI receptor. II. Stable expression in Chinese hamster ovary cells reveals the interaction with three major signal transduction pathways. J. Neurochem. 62, 519-27. 

Manya, H., et al. (2000). Comparative study of the asparagine-linked sugar chains of human lipocalin-type prostaglandin D synthase purified from urine and amniotic fluid, and recombinantly expressed in Chinese hamster ovary cells. 

Nestmann ER, Douglas GR, Matula TI, Grant CE, Kowbel DJ (1979) Mutagenic activity of rhodamine dyes and their impurities as detected by mutation induction in Salmonella and DNA damage in Chinese hamster ovary cells. Cancer Res 39 4412-4417... 

Davis, S.J., Ward, H.A., Puklavec, M.J. et al. (1990) High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants. The Journal of Biological Chemistry, 265 (18), 10410-10418. 

The equivalent of the tryptic fragment of human transferrin receptor has been expressed in Chinese hamster ovary cells and its structure determined at a resolution of 0.32 nm (Lawrence et ah, 1999). The asymmetric unit of the crystals contains four transferrin receptor dimers. Interpretable electron density is found for the entire tryptic fragment except for Arg-121 at the amino terminus, and density is also seen for the first N-acetylglucosamine residue at each of the N-glycosylation sites. The transferrin receptor monomer is made up of three distinct domains, organized such that the dimer is butterfly shaped (Figure 5.10, Plate 7). The likely orientation of the dimer with respect to the plasma membrane has been assigned on the basis of the... 

Chen, H., et al. Molecular cloning and functional expression of a chicken intestinal peptide transporter (cPepTl) in Xenopus oocytes and Chinese hamster ovary cells. J. Nutr. 2002, 132, 387-393. 

Covitz, K. M., G. L. Amidon, and W. Sadee. Human dipeptide transporter, hPEPTl, stably transfected into Chinese hamster ovary cells. Pharm. Res. 1996, 13, 1631-1634. 

Juliano, R. L., Ling, V., A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants, Biochim. Biophys. Acta 1976, 455, 152-162. 

Some drugs with low intrinsic permeability achieve acceptable oral bioavailability because they are substrates for uptake transporters, which normally function in nutrient uptake. The most prominent example is the peptide transporter, PepTl, which is active toward peptidomimetic antibiotics such as cephalexin, the antiviral agent valacyclovir [24] and other drugs. PepTl is natively expressed in Caco-2 cells, and adenovirus transduction has been used to increase PepTl expression levels [25]. However, the expression of PepTl was not polarized in this system and this expressed system appears to be of limited value as an improved screening model. PepTl has also been expressed in Chinese hamster ovary cells and a variety of other mammalian systems [26, 27]. 

McClean, S., Whelan, R. D., Hosking, L. K., Hodges, G. M., Thompson, F. H., Meyers, M. B., Schuurhuis, G. J., Hill, B. T., Characterization of the P-glycoprotein over-expressing drug resistance phenotype exhibited by Chinese hamster ovary cells following their in-vitro exposure to fractionated X-irradiation, Biochim. Biophys. Acta 1993, 1177, 117-126. 

Avidor-Reiss T, Bayewitch M, Levy R, Matus-Leibovitch N, Nevo I, Vogel Z. Adenylylcyclase supersensitization in mu opioid receptor transfected Chinese hamster ovary cells following chronic opioid treatment. J Biol Chem 1995 270 29732-29738. 

Human delta opioid receptor Functional studies on stably transfected Chinese hamster ovary cells after acute and chronic treatment with the selective non-peptidic agonist SNC-80. J Pharmacol Exp Ther 1996 278 1083-1089. 

Guerrant RL, Brunton LL, Schnaitman RC, Rebhun LI, Gilman AG Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology A rapid, sensitive in vitro assay for the entertoxins of Vibrio cholerae and Escherichia coli. Infect Immun 1974 10 320-327. 

Additional studies were performed with eukaryotic cell systems (i.e. Chinese hamster ovary cells, rat bone mar-... 

Expression of the cloned receptors in Chinese hamster ovary cells, other mammalian cells and Xenopus oocytes has demonstrated differential coupling of these receptors to cellular responses. In general the Mb M3 and M5 receptors regulate phosphoinositide hydrolysis by stimulating PLC. This occurs through selective coupling of the receptor to the pertussis-toxin-insensitive G protein Gq/11,... 

The selectivity in muscarinic receptor coupling is not, however, absolute. Overexpression of receptors or of particular G proteins supports interactions that may differ from those described above. For example, M2 receptors expressed in Chinese hamster ovary cells not only inhibit adenylyl cyclase but also can stimulate phosphoinositide hydrolysis through a pertussis-toxin-sensitive G protein [52] this is not seen, however, when M2 receptors are expressed in Y1 cells. These findings indicate that caution must be exercised in interpreting data obtained when receptors are expressed, often at high levels, in cells in which they normally do not function. 

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