Chemisorption, immobilization

The observed distribution can be readily explained upon assuming that the only part of polymer framework accessible to the metal precursor was the layer of swollen polymer beneath the pore surface. UCP 118 was meta-lated with a solution of [Pd(AcO)2] in THF/water (2/1) and palladium(II) was subsequently reduced with a solution of NaBH4 in ethanol. In the chemisorption experiment, saturation of the metal surface was achieved at a CO/Pd molar ratio as low as 0.02. For sake of comparison, a Pd/Si02 material (1.2% w/w) was exposed to CO under the same conditions and saturation was achieved at a CO/Pd molar ratio around 0.5. These observations clearly demonstrate that whereas palladium(II) is accessible to the reactant under solid-liquid conditions, when a swollen polymer layer forms beneath the pore surface, this is not true for palladium metal under gas-solid conditions, when swelling of the pore walls does not occur. In spite of this, it was reported that the treatment of dry resins containing immobilized metal precursors [92,85] with dihydrogen gas is an effective way to produce pol-5mer-supported metal nanoclusters. This could be the consequence of the small size of H2 molecules, which... 

SCHEME 1 Modification of double-stranded DNA with organosulfur precursor and immobilization on An surface via chemisorption. 

The first STM evidence for the facile transport of metal atoms during chemisorption was for oxygen chemisorption at a Cu(110) surface at room temperature 10 the conventional Langmuir model is that the surface substrate atoms are immobile. The reconstruction involved the removal of copper atoms from steps [eqn (1)], resulting in an added row structure and the development of a (2 x 1)0 overlayer [eqn (2)]. The steps present at the Cu(llO) surface are... 

At a Pd(l 11) surface at room temperature, the chemisorption state is disordered when the NO pressure is less than 3 x 10-6 Torr with very noisy STM images due to the high mobility of the adsorbed molecules.14 With increasing pressure (and coverage), the c(4 x 2) state, which is reversible, is locked-in and immobile. The adsorption at lower temperatures (150-200 K), where the coverage exceeds that at room temperature, the c(4 x 2) state coexists with a p(2 x 2) and a c(8 x 2) phase the latter is only present when it coexists with the c(4 x 2) and p(2 x 2) states. 

The use of dispersed or immobilized transition metals as catalysts for partial hydrogenation reactions of alkynes has been widely studied. Traditionally, alkyne hydrogenations for the preparation of fine chemicals and biologically active compounds were only performed with heterogeneous catalysts [80-82]. Palladium is the most selective metal catalyst for the semihydrogenation of mono-substituted acetylenes and for the transformation of alkynes to ds-alkenes. Commonly, such selectivity is due to stronger chemisorption of the triple bond on the active center. 

In addition to phosphine ligands, a variety of other monodentate and chelating ligands have been introduced to functionalized polymers [1-5]. For example, cyclo-pentadiene was immobilized to Merrifield resins to obtain titanocene complexes (Fig. 42.13) [102]. The immobilization of anionic cyclopentadiene ligands represents a transition between chemisorption and the presently discussed coordinative attachment of ligands. The depicted immobilization method can also be adopted for other metallocenes. The titanocene derivatives are mostly known for their high hydrogenation and isomerization activity (see also Section 42.3.6.1) [103]. 

In a similar approach Riihe et al. [279] reported the preparation ofpoly(2-oxazoline) brushes by the grafting onto as well as grafting from method. For LCSIP of 2-ethyl-2-oxazolines silane functionalized undecane tosylate was first prepared and then immobilized on the substrate surface. SIP resulted in PEOx layers with thickness close to 30 nm. PEOx brushes were prepared by chemisorption of PEOx disulfides onto gold substrates. Preliminary static and dynamic swelling experiments are reported for these brushes. However, later observations [243] contradicted these findings. 

Other methods that are related to affinity chromatography include hydrophobic interaction chromatography and thiophilic adsorption. The former is based on the interactions of proteins, peptides, and nucleic acids with short nonpolar chains on a support. This was first described in 1972 [113,114] following work that examined the role of spacer arms on the nonspecific adsorption of affinity columns [114]. Thiophilic adsorption, also known as covalent or chemisorption chromatography, makes use of immobilized thiol groups for solute retention [115]. Applications of this method include the analysis of sulfhydryl-containing peptides or proteins and mercurated polynucleotides [116]. 

Lunsford et al. (202) used trimethylphosphine as a probe molecule in their 31P MAS NMR study of the acidity of zeolite H-Y. When a sample is activated at 400°C, the spectrum is dominated by the resonance due to (CH3)3PH+ complexes formed by chemisorption of the probe molecule on Bronsted acid sites. At least two types of such complexes were detected an immobilized complex coordinated to hydroxyl protons and a highly mobile one, which is desorbed at 300°C. (see Fig. 45)... 

There are three principal approaches used for the immobilization of electroactive substances onto surfaces chemisorption, covalent bonding, and film deposition. 

In this part we will describe recent achievements in the development of biosensors based on DNA/RNA aptamers. These biosensors are usually prepared by immobilization of aptamer onto a solid support by various methods using chemisorption (aptamer is modified by thiol group) or by avidin-biotin technology (aptamer is modified by biotin) or by covalent attachment of amino group-labeled aptamer to a surface of self-assembly monolayer of 11-mercaptoundecanoic acid (11-MUA). Apart from the method of aptamer immobilization, the biosensors differ in the signal generation. To date, most extensively studied were the biosensors based on optical methods (fluorescence, SPR) and acoustic sensors based mostly on thickness shear mode (TSM) method. However, recently several investigators reported electrochemical sensors based on enzyme-labeled aptamers, electrochemical indicators and impedance spectroscopy methods of detection. 

Fig. 33.2. Schematic picture of the methods of immobilization of the aptamers (A) chemisorption. Biotinylated aptamer is immobilized on a surface covered by (B) avidin, (C) neutravidin and (D) avidin on a surface of poly(amidoamine) dendrimers (PAMAM). (E) Aptamer is immobilized on carbon nanotubes activated by Tween 20 (it is partially adapted from Ref. [39] with permission of American Chemical Society).

This also holds for the other mechanism—known as the Langmuir-Hinshelwood mechanism—where the two reactant molecules have to be chemisorbed side by side. Such pairs can be formed statistically by the molecules hitting the surface and being bound by chemisorption forces at the very spots where they hit (localized adsorption) or they may result from collisions of the molecules moving along the surface of the catalyst-(mobile adsorption). It may again be stated that the formation of pairs in the case of immobile localized adsorption is more favorable for reaction than in the case of mobile adsorption. 

Analyte recognition species for biosensors chemisorption, 9 immobilization, 8-12... 

Preparation of metal oxide thin film by means of stepwise absorption of metal alkoxide has been carried out in the past for the activation of heterogeneous catalysts [13]. For example, Asakura et al. prepared one-atomic layer of niobium oxide by repeating chemisorption of Nb(OEt)5 on silica beads. The catalyst obtained by immobilizing platinum particles on a niobum oxide layer showed improved reactivity for hydrogenation of ethylene in comparison with... 

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