Chemical Proteomics for Kinases KinaTor

Cell extracts in lanes 2 to 5 and lanes 6 to 9 represent cells transfected with wild-type EGF-R and EGF-RT766M, respectively. 

Affinity chromatography using immobilised protein kinase inhibitors 

In the past, immobilization of compounds and subsequent affinity chromatography has been tried at many companies and at universities. So far, with a few exceptions coming from the field of the CDK inhibitors, purvalanol and paullones [54-56], the technology has not yet delivered, because the following factors were not considered  

Identification of several new protein kinase targets of the p38 inhibitor SB203580 

In summary, the KinaTor technology appears to be extremely potent for potentially revealing all binding partners of biologically active kinase inhibitors. Transmembrane domain proteins bind, as well as lipid kinases and other nucleotidebinding proteins, such as heat-shock proteins and oxidoreductases (unpublished data). So far, there are no limitations. 

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