Changing work methods/patterns

These problems were overcome by immobilizing the enzyme. Since the usual methods for immobilization of laccase did not work, we adopted a new method, details of which will be described elsewhere (29). Reasonable measurements were possible with this technique. Typical patterns of laccase activity could be monitored via the changes of absorbance of 2,6 DMP and syringaldazine. When the reaction took place in organic solvents, the absorption spectra of the products were similar to those obtained for the same reaction in buffer. Furthermore, the catalytic action of the T. versicolor laccase followed Michaelis-Menten-kinetics in most of the organic solvents which were tested (see Table II for specific examples). 

The current work is focused on application of thermogravimetry in qualitative characterization of structure of MCM-41 samples prepared using the hydrothermal restructuring method [22-25] and direct synthesis procedure involving hexadecyldimethylamine as an expander [25,26]. It is demonstrated that the weight-change patterns of as-synthesized samples provide qualitative information about phase purity, stability upon calcination and the resulting structure of the calcined samples. 

As is known from the work of Domschke and Oelmann143,144 dimethyl 2-aminofu-marate reacts with quinones to give aminocoumarins, whereas with BF3 in ether the corresponding N-monosubstituted enaminones substitution pattern is changed and the normal Nenitzescu products are formed, from which 2,3-unsubstituted 5-hydroxyindoles are accessible (equation 94). This method is not well known in the literature, but it was recently used145. 

Political and scientific attention has recently turned towards investigating substances that possess hormonal activity or alter patterns of hormone effects. Not only does detecting such changes in organisms prove to be extremely complicated, these endocrine disruptors can be active at very low doses. Considerable work for the standardisation of endocrine disruptor test methods has been undertaken at EU and international levels, but there is currently little agreement on how to test for such effects [123]. 

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