Chandler loop

The Chandler loop technique allows one to produce in vitro thrombi in a moving column of blood (Chandler 1958). The thrombi generated in the Chandler device show morphology very similar to human thrombi formed in vivo (Robbie et al. 1997) with platelet-rich upstream sections ( white heads ) that are relatively resistant to t-PA-mediated thrombolysis in contrast to the red blood-cell-rich downstream parts ( red tails ) (Stringer et al. 1994). 

Time to occlusion of the tube by the thrombus establishes a definite end point in this system. 

Stringer et al. (1994) used this method to determine the influence of an anti-PAI-1 antibody (CLB-2C8) on the 

Chandler AB (1958) In vitro thrombotic coagulation of the blood. A method for producing a thrombus. Lab Invest 7 110-114 

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Robbie LA, Young SP, Bennett B, Booth NA (1997) Thrombi formed in a Chandler loop mimic human arterial thrombi in structure and PAI-1 content in distribution. Thromb Haemost 77 510-515... 

Stringer HAR, Van Swieten P, Heijnen HFG et al. (1994) Plasminogen activator inhibitor 1 released from activated platelets plays a key role in thrombolysis resistance. Studies with thrombi generated in the Chandler loop. Arte-rioscler Thromb 14 1452-1458... 

Figure 2.26 Schematic presentation of in vitro blood loop test Chandler, hemobile, and roUer pump.

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