Chemoenzymatic synthesis cephalosporin

Terreni, M., Pagani, G., Ubiali, D., et al. 2001. Modulation of penicillin acylase properties via immobilization techniques One-pot chemoenzymatic synthesis of Cephamandole from Cephalosporin C. Bioorganic and Medicinal Chemistry Letters, 11 2429-32. 

Justiz, O. H. Fernandez-Lafuente, R. Guisan, J. M. Negri, P. Pagani, G. Pregnolato, M. Terreni, M., One-Pot Chemoenzymatic Synthesis of 3 -Functionalized Cephalosporins (Cefazolin) by Three Consecutive Biotransformations in Fully Aqueous Medium. / Org. Chem. 1997, 62, 9099. 

A one-pot chemoenzymatic synthesis of 3 -functionalized cephalosporin C or cepha-zolin involving three consecutive biotransformations has been reported [16]. The enzymatic deacylation of cephalosporin C in one batch was performed by the use of D-amino acid oxidase (DAO) from Trigonopsis variabilis, glutaryl acylase (GA) from Acetobacter sp., and a continuous flow of oxygen. DAO catalyzed the oxidative deamination of the a-... 

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