Cephaelin Chamber saturation

Note Under the conditions employed emetine and cephaeline were not well separated but there was good resolution of the subsidiary alkaloids of the ipecacuanha tincture (Fig. 1). The separation and quantitative determination of the main alkaloids (Fig. 2) can be carried out under the following conditions Ascending, one-dimensional development in a trough chamber with chamber saturation layer HPTLC plates Silica gel 60 (Merck) mobile phase dichloromethane — methanol — ammonia solution (25%) (34+6+1) migration distance 6 cm running time 13 min h/ f cephaeline 65-70 emetine 75-80. 

Isoquinoline Alkalmds 12] Method Ascending, one-dimensional double development in a trough chamber with chamber saturation. (A) and an extract of Ipecacuanhae Radix (B) cephaeline (1), emetine (2). 

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