Centrifuge pulse

Figure 10.4 (a) Illustration of a centrifuge pulse. The vector of linearly polarized laser field E... 

Pellet the glass milk by centrifugation (pulse to over 12,000g and release). Discard the supernatant. 

The centrifugal pump is a versatile unit in the process plant, since its ease of control, non-pulsing flow, pressure limiting operation fits many small and large flow systems. 

Preselection of a suitable extractor type on the basis of available information and testing at pilot scale Establishment of suitable operating conditions as, for example, the pulse intensity of pulsed extractors or the speed of rotation of centrifugal extractors Determination of the flooding point... 

Resuspend the phage pellet in 2 mL PBS, and clarify by a short pulse of centrifugation in a microfuge. 

The graph shows E. coli labeled with radioactive thymidine for a short pulse (10 s) followed by a chase with an excess of nonradioactive thymidine. The DNA is then extracted and centrifuged in alkaline sucrose gradients (under high pH conditions the DNA denatures). Explain what these data imply, and interpret these results in light of our current model for DNA replication. 

---