Centrifugation, equilibrium density gradient

Illustration showing separation by equilibrium-density-gradient centrifugation. The homogeneous mixture in (a) separates into three bands (b) after applying centrifugal force. 

Fig. 2. Separation of organelles by equilibrium density-gradient centrifugation.

W. Szybalski, Use of Cesium Sulfate for Equilibrium Density Gradient Centrifugation, in Methods in Enzymology, Vol. 12B (L. Grossman and K. Moldave, Eds.), Academic Press, New York, 1968, p. 330. 

Equilibrium density-gradient centrifugation, which separates cellular components according to their densities, can further purify cell fractions obtained by differential centrifugation. 

H9. Hardingham, T. E., and Muir, H., Biosynthesis of proteoglycans in cartilage slices. Fractionation by gel chromatography and equilibrium density-gradient centrifugation. Biochem. J. 126, 791-803 (1972). 

Figure 4.2.4A. Equilibrium density gradient in a centrifuge due to CsCl solution.

Szybalski, W. Use of cesium sulfate for equilibrium density-gradient centrifugation. In Methods in Enzymology, Vol. XIIb. p. 330-360. New York and London Academic Press 1968. 

The RI-RNA obtained contained 2.3% of the total newly incorporated radioactivity and 40% of the total input label recovered by phenol extraction. Analysis by equilibrium density gradient centrifugation revealed properties expected for RI-RNA (Fig. 19a). 

Equilibrium sedimentation technique working with a multi-component solvent forming a density gradient in a centrifugal field... 

DNA extracted and centrifuged to equilibrium in CsCl density gradient... 

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