Centrifugation caesium chloride density gradient

Figure 13.7 Caesium chloride density gradient centrifugation for (a) the separation of DNA from RNA and protein and (b) the separation of linear DNA and supercoiled DNA.

When total DNA is subjected to high speed density gradient centrifugation in caesium chloride, a large band of nuclear DNA and one or more small satellite bands are usually observed. Up to four satellite DNA bands have been detected in... 

For zonal density gradient centrifugation there are a number of media that can be used, however the most common of these is sucrose whereas the most common medium for isopycnic density gradient centrifugation is caesium chloride (CSCI2). 

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