Cellobiose dehydrogenase acceptors

Table 111. Apparent Kinetic Constants of Cellobiose Dehydrogenase from S. rolfsii for Several Electron Acceptors...

Cellobiose dehydrogenase (CDH) preferentially oxidizes cellobiose, the product of cellulose hydrolysis by cellulases. A variety of substrates can be used as electron acceptors, including quinones, transition metals, phenoxyl radicals, and O2 (Figure 1C, 14). A possible secondary reaction is the production of Fenton reagent resulting in the hydroxyl radical. 

Cellobiose dehydrogenase oxidizes cellobiose to cellobionolactone and transfers the electrons to a wide range of electron acceptors. The oxidized enzyme is... 

For practical implementation the use of parallel cascades is of great value for cofactor recycle. For example, van Hecke and coworkers [45] report the synthesis of lactobi-onic acid from lactose using cellobiose dehydrogenase. The enzyme requires an electron acceptor, and using ABTS and laccase in a parallel cascade, such a system was effectively operated and modeled. 

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