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Cell lysates sequential extraction

Methods for cell and sub-cellular fractionation include immuno-isolation, electromigration e.g. free flow electrophoresis), flow cytometiy, density gradient isolation of organelles and sequential extraction. The use of some of these approaches to investigate the mitochondrial and myofibrillar sub-proteomes of the heart will be described in Sections 3.4 and 3.6 respectively. A particular problem in proteomic analysis of the heart is the diversity of cell types that are present. Proteomic profiles of total myocardial lysates are dominated by the proteins present in cardiac myocytes, but such samples... [Pg.26]

Sequential extraction of cells to recover lysates enriched in cytosolic and nuclear proteins 268 Controlled cell lysis to produce a post-nuclear supernatant homogenate of cytoplasmic proteins 269... [Pg.507]

Sequential extraction of cells to recover lysates enriched in cytosolic and nuclear proteins... [Pg.268]

See other pages where Cell lysates sequential extraction is mentioned: [Pg.106]    [Pg.67]    [Pg.540]    [Pg.30]   


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