Endocytosis caveolae-like

Cave olae -mediated and caveolae-like endocytosis... 

Caveolae-Mediated Endocytosis and Caveolae-Like Endocytosis Pharmacological Inhibitors... 

Keep in mind that exocytosis might occur during sample preparation (e.g., preparing cells for flow cytometric analysis). After the experiment (and prior to analysis) cells should be kept below 4°C to block all active processes (such as exocytosis). Exocytosis might also be blocked with NEM (see section Caveolae-Mediated Endocytosis and Caveolae-Like Endocytosis Pharmacological Inhibitors ). 

Fig. 3. Model of the life cycle of prions. PrP is synthesized in the rough endoplas-matic reticulum (ER), and after passing through the secretory pathway including the Golgi and secretory vesicles, reaches the surface of a PrP infectable cell where it is anchored via a glycosylphosphatidyl inositol (GPI) moiety. Endocytosis of PrP and possibly PrP via clathrin coated vesicles could be mediated by the 37 kDa laminin receptor precursor (LRP). The uptake of the infectious agent could also be LRP independent. The conversion of the internalized PrP to PrP is thought to take place in the endo-somes, lysosomes, or endolysosomes. Molecular chaperones could be involved in this conversion process. PrP replication and aggregation can occur in neuronal cells of the brain but also in the cells constituting the lymphoreticular system. Alternatively, endocytosis and conversion of PrP into PrP could happen in caveolae-like domains (CLDs).

Caveolae-mediated endocytosis is involved in viral transfection. This route can therefore be used for the delivery of oncolytic genetic materials by a viral vector [120]. Macropinocytosis is a relatively non-specific process which allows uptake of large particles up to the micron size range [121]. It is likely useful for the delivery of systems like solid lipid nanoparticles and PLN, which are... 

The involvement of clathrin-coated pits in endocytosis of PrP is surprising, as GPI-anchored proteins like PrP " lack a cytoplasmic domain that could interact directly with adapter proteins and clathrin. Indeed, it has heen speculated that other GPI-anchored proteins are excluded from coated pits and are internalized via other surface invaginations called caveolae (Anderson, 1993). We find, however, that caveolae are not responsible for internalization of PrP " in neuronal cells, as these cells lack morphologically recognizable caveolae and do not express caveolin (Shyng etal., 1994). 

Kumano-Kuramochi M, Xie Q, Kajiwara S, Komba S, Minowa T, Machida S. Lectin-like oxidized LDL receptor-1 is palmitoylated and internalizes ligands via caveolae/ raft-dependent endocytosis. Bwchen j al nd w h sical esearcli Co 2013 434 594-599. 

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