Catharanthus roseus brassinosteroids

Suzuki, H., Inoue, T., Fujika, S., Saito, T., Takatsuko, S., Yokota, T., Murofushi, N., Yanagisawa, T. and Sakurai, A. (1995) Conversion of 24-methylcholesterol to 6-oxo-24-methylcholestanol, a putative intermediate of the biosynthesis of brassinosteroids, in cultured cells of Catharanthus roseus. Phytochemistry, 40,1391-7. 

The extract of cells of Catharanthus roseus were partially purified by reverse phase HPLC and were analyzed by GC-MS, disclosing that the major brassinosteroids in the cells are brassinolide and castasterone (5). Change of the contents of brassinolide and castasterone during cell growth was pursued by GC-SIM using D brassinolide and... 

Identification of Brassinosteroids Produced by Crown Gall Cells of Catharanthus roseus... 

We express our thanks to Prof. K. Syono of Department of Pure and Applied Sciences, The University of Tokyo for his collaboration to supply us cell lines from Catharanthus roseus and rice, to Prof. T. Yokota of Department of Bioscience, Teikyo University for his collaboration in identification of the brassinosteroids. We also wish to thank Dr. K-H. Park, Mrs S. Nakagawa, Miss M. C. Cifuentes-Barreto, Miss M. Ohtsuka and Miss M. Yamamoto for their collaboration in these studies at our laboratories. 

Fujioka S, Noguchi T, Watanabe B, Takatsuto S, Yoshida S (2000) Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus. Phytochemistry 53 549-553... 

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