Catalyzed lodination

The following protocol describes the use of Iodogen for the radioiodination of proteins and peptides. 

Caution Handle all radioactive substances according to the radiation safety regulations instituted at each facility approved to handle such materials. Use adequate precautions to protect personal safety and the environment from contamination. Dispose of radioactive waste by following approved guidelines. 

In a fume hood, dissolve 10-100 pig of Iodogen (Thermo Fisher) in 100-500 pil of chloroform, methylene chloride, or DMSO. The use of 10 pig of Iodogen per 100 pig of protein or 107 cells to be iodinated will result in good incorporation yields. 

Rinse the plated reaction vessel once with sample buffer to remove any loose particles of Iodogen that may not be strongly adhered to the surface of the glass. 

Add carrier-free Na125I to the reaction vessel in a ratio of about 500 piCi per 100 pig protein. 

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Lactoperoxidase-Catalyzed lodination as a Tool for Investigation of Proteins... 

Lactoperoxidase-Catalyzed lodination of Plasma Membrane Lipids... 

Tyrosine Residues Accessible Polypeptide to Lactoperoxidase-catalyzed lodination of Intact Virions ... 

Oxidative Cleavage of Tryptophanyl Peptide Bonds during Chemical- and Peroxidase-catalyzed lodinations. J. Biol. Chem. 249, 1946-1952 (1974). 

Crich and Rumthao reported a new synthesis of carbazomycin B using a benzeneselenol-catalyzed, stannane-mediated addition of an aryl radical to the functionalized iodocarbamate 835, followed by cyclization and dehydrogenative aromatization (622). The iodocarbamate 835 required for the key radical reaction was obtained from the nitrophenol 784 (609) (see Scheme 5.85). lodination of 784, followed by acetylation, afforded 3,4-dimethyl-6-iodo-2-methoxy-5-nitrophenyl acetate 834. Reduction of 834 with iron and ferric chloride in acetic acid, followed by reaction with methyl chloroformate, led to the iodocarbamate 835. Reaction of 835 and diphenyl diselenide in refluxing benzene with tributyltin hydride and azobisisobutyronitrile (AIBN) gave the adduct 836 in 40% yield, along with 8% of the recovered substrate and 12% of the deiodinated carbamate 837. Treatment of 836 with phenylselenenyl bromide in dichloromethane afforded the phenylselenenyltetrahydrocarbazole 838. Oxidative... 

Lastly, a formal synthesis of almotriptan (5) has been published by the Bosch group in Spain using an intramolecular Heck approach (Scheme 23). lodination of aniline 58 with bis(pyridine)iodonium(I) tetrafluoroborate followed by reaction with trifluoroacetic anhydride (TFAA) gave the o-iodotrifluoroacetanilide 66. Alkylation of 66 with methyl 4-bromocrotonate afforded 67, which underwent palladium catalyzed... 

Dirhodium(ll)-Catalyzed C(sp )-H Amination Using lodine(lll) Oxidants... 

Scheme 14 lodination of a stable persilapropellane to a hexaiodocyclopentasilane Equation 7. Lewis acid catalyzed rearrangement/cyclization of an oligosilane. 

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