Caseinolytic protease

This enzyme [EC 3.4.21.92], also referred to as endopep-tidase Ti and caseinolytic protease, catalyzes the hydroly-... 

Some answers may be obtained from smaller bacterial, mitochondrial, and chloroplast ATP-dependent proteases. Cells of E. coli contain at least nine proteases, which have been named after the musical scale as Do, Re, Mi, Fa, So, La, Ti, Di, and Ci.450 451 Most are serine proteases but two, Ci and Pi, are metalloproteins. Protease La (Lon protease, encoded by gene Ion) has attracted particular attention because the hydrolysis of two molecules of ATP occurs synchronously with cleavage of a peptide linkage in the protein chain 452 This enzyme, as well as protease Ti (more often called CIp, for caseinolytic protease),451453 454 is ATP-dependent.451... 

Actinidin also hydrolyzes a-casein and (3-casein in milk (Nishiyama and Oota, 2002 Yamaguchi et al, 1982). Bachmann and Farah (1982) demonstrated the occurrence of a bitter taste in mixtures of milk proteins and raw kiwifruit, which was attributed to a caseinolytic protease in kiwifruit splitting casein into bitter peptides. 

Bernard, D. et al. Analysis of proteins with caseinolytic activity in a human SC extract revealed a yet unidentified cysteine protease and identified the so called SC thiol protease as Cathepsin L2, J. Invest. Dermatol., 120, 592-600, 2003. 

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