Cascade Reactions Involving AAOs

AAOs were also combined with other biocatalysts in various cascade reactions [66, 120]. A couple of processes involving AAOs were established providing enantiomerically pure building blocks in the form of a multienz3mie network. For example, o-methionine was effectively converted to L-methionine using four different enzymes in one pot oxidation of the o-amino acid to the corresponding a-keto acid was achieved with d-AAO originating from Arthrobacter protophormiae, 

Stereoinversion of d- into t-methionine by a muitienzyme network, combining a d-AAO with L-phenyiaianine dehydrogenase (i-PheDH), cataiase, and an FDH in one pot. 

Enzymatic cascade providing access to cyclic amino acid (AA) derivatives by the combination of AAO with /V-methyi amino acid dehydrogenase (NMAADH) starting either from a linear or cyclic AA. 

Notably, the reaction was initially performed on a liter scale but was then scaled up to convert 1 kg of substrate [124]. 

2-amino-3-(7-methyl-1//-indazol-5-yl)propanoic acid N J N H [I ] C02H 

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