Cascade Processes, Including Biocatalyzed Reductive Amination Steps

Cascade Processes Including Biocatalyzed Reductive Amination Steps 

From the late 1980s, muitienzymatic cascade processes aiming at the synthesis of optically pure amino acids and amines by asymmetric synthesis and kinetic resolution have been thoroughly investigated [2]. 

Several multienzymatic processes employing transaminases have been developed for the production of enantiopure amines and amino acids. 

Finally, great effort has been recently devoted to the exploitation of the so-called co-transaminases (co-TAs) for the preparation of optically pure amines [31]. These biocatalysts are generally pyridoxal-5 -phosphate (PLP)-dependent enzymes and are capable of performing reductive amination readions without using either an a-amino add as amine donor or an a-keto add as amino acceptor. Therefore, they find several applications in the asymmetric synthesis of nonracemic a hiral primary amines, and enzymes with different substrate spedfidty are currently available also from commercial sources. 

In most cases, co-TAs can use alanine as amine donor and produce pyruvate as by-produd. This finding greatly simplified the successful exploitation of this dass of enzymes as different methods can be used for either the removal of pyruvate or the recyding of alanine, thus permitting to shift the readion equilibrium toward the formation of the amine of interest. 

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Cascade Processes Including Biocatalyzed Reductive Amination Steps I 297... 

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