Carnitine dehydratase

Synthesis of Carnitine Catalyzed by Carnitine Dehydratase in Whole Cells (E.C. 4.2.1.89)[8 46 97-991... 

L-Camitine is used in infant health, sport and geriatric nutrition. The biotransformation is catalyzed by carnitine dehydratase in whole cells (Fig. 19-36). (R)-camitine is produced with > 99.5% conversion of butyrobetaine and > 99.5% ee. The mutant strain has blocked the L-carnitine dehydrogenase and excretes the accumulated product. The purified enzyme could not be used for the biotransformation because of its high instability. Apart from usual batch fermentations, continuous production... 

E = carnitine dehydratase, whole cells from Escherichia coli... 

In a totally different type of study, through a novel use of the [AuCU] salt, (2i ,3i )-L-(—)-[2-D]carnitine tetrachloroaurate, Bau et al. have used single erystal nentron diffraction to determine the absolute stereochemistry of the erotonobetaine to earnitine transformation as it is catalyzed by L-carnitine dehydratase from Escherichia coli. 

Carnitine dehydratase/bile acid-inducible protein (1461503-1462636)... 

Bau, R., Schreiber, A., Metzenthin, T., Lu, R.S., Lutz, E, Klooster, W.T., Koetzle, T.E, Siem, H., Kleber, H.R, Brewer, E, and Englard, S., Neutron diffraction structure of (2R,3R)-L-(-)-[2-D] carnitine tetrachloroaurate, [(CH3)3N-CH2-CHOH-CHD-COOH]+[AuCl4] determination of the absolute stereochemistry of the crotonobe-taine-to-carnitine transformation catalyzed by L-carnitine dehydratase from Escherichia coli. J. Am. Chem. Soc., 119, 12055-12060, 1997. 

---