Carboxypeptidase tissue distribution

Specialized histochemical, immunohistochemical (IHC), and other tissue section-based molecular techniques (e.g., in situ hybridization (ISH)) can be used to better characterize the nature of the injury, cell types affected and its relationship to test article distribution and/or its intended target. The distribution of digestive enzymes or their proenzymes in the zymogen granules of acinar cells can be demonstrated using IHC markers for trypsin, chymotrypsin, carboxypeptidases A and B, lipase, amylase, elastase, DNase, and RNase (Cattley et al., 2013). Cytokeratin IHC markers can be used to differentiate epithelial components of the pancreas, most notably the cells of the ductal system. Amylase and MISTI have used been as IHC markers to confirm acinar cell differentiation experimentally. Trypsinogen activation peptide (TAP), a peptide released from trypsinogen when trypsin is activated, can be used as an IHC marker for intracellular activation... 

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