Carbonic Anhydrase Inhibition in vitro

CO2 flow rate is adjusted to 30(45) ml/min. The following solutions are added to the reaction vessel  

Tu = (uncatalyzed time) = time for the color change to occur in the absence of enzyme 

Tj = enzyme rate in the presence of various concentrations of inhibitor. 

Percent inhibition of carbonic anhydrase is calculated according to the following formula  

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