Carbodiimides liposome conjugation

Modification of the protein amine groups is the procedure most frequently used to produce antibody-liposome conjugates. Early procedures used crosslinking agents, such as l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (27,28) in the presence of preformed liposomes containing a lipophilic carboxylic acid. Condensing agents like these tend to form protein-protein polymers. Control of these reactions is typically difficult and complex, and as a result separation of the liposomes from protein polymers is a... 

Figure 22.22 A protein may be conjugated with a liposome-containing PE groups using a carbodiimide reaction with EDC.

Figure 1 Conjugation reactions for coupling peptides to the surface of preformed liposomes. Functionalized lipophilic anchors were incorporated into liposomes and reacted with the peptides in aqueous media. Reactive endgroup functions (A) maleimide (B) bromo- or iodoacetyl (C) 2-pyridyldithio (D) carboxylic acid (E) />-nitrophenyl carbonate and (F) hydrazide. Abbreviations EDC, l-ethyl-3-(3-dimethylaminopropyl)carbodiimide NHS, 7V-hydroxysuccinimide.

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