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Carbodiimide hapten coupling procedure

The coupling of carboxyl-containing compounds to amino groups of the polypeptide carrier is usually performed with an excess of carboxyl groups and an equivalent amount of the water-soluble carbodiimide around pH 5 at room temperature. In some cases where the hapten is not water soluble, it is usually dissolved in DMF or dioxane. In such cases it is also possible to prepare the hydroxysuccinimide ester using dicyclo-hexylcarbodiimide and couple this ester directly to the carrier. The experimental procedure will be demonstrated by two examples. [Pg.156]

See other pages where Carbodiimide hapten coupling procedure is mentioned: [Pg.829]    [Pg.325]    [Pg.639]    [Pg.754]    [Pg.755]    [Pg.889]    [Pg.849]    [Pg.448]    [Pg.449]    [Pg.579]    [Pg.95]    [Pg.153]    [Pg.159]    [Pg.204]    [Pg.547]    [Pg.428]    [Pg.429]    [Pg.559]   
See also in sourсe #XX -- [ Pg.76 , Pg.156 ]



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Carbodiimid

Carbodiimide

Carbodiimide coupling

Carbodiimide coupling procedure

Carbodiimids

Coupling procedures

Hapten

Haptenation

Haptene

Haptens

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