Carbamate linkage Carbohydrate

Traditionally in biochemistry (e.g. for affinity chromatography), enzymes have been covalently linked to carbohydrates such as agarose and dextran (Sepharose, Sephadex). The most widely used activation method is the cyanogen bromide (CNBr) method, yielding isourea and imidocarbonate functionalities that react with amines on the enzyme to produce N-substituted carbamate linkages. 

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