Capture probes templates

Site-Selective Attachment of Capture Probes onto Chemical Templates. .. 107... 

Figure 4 Sensitive protein detection using the RCA (rolling circle amplification) immunoassay chip and solid-phase direct fluorescence detection. The chip is divided into 16 teflon wells, each containing an array of 256 antibodies as probes. When a protein, represented by the blue square, is captured by one of the probes, it can be recognized using a second, biotinylated antibody (red), which is subsequentiy detected by a tertiary universal antibody connected to a circular oligonucleotide. A strand-displacing DMA polymerase can use this circular template, generating a long concatemer containing many fluorescent labels.

Figure 9 Selected examples for (a) colorimetric and (b) CD probes that respond to covalent capture and ion-templated self-assembly, respectively.

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