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Capillary isoelectric focusing ampholyte buffers

Figure 12.13. CIEF of cytochrome c (peak 1, pi 9.6), chymotrypsinogen A (peak 2, pi 9.0) and myoglobin (peaks 3 and 4, pi 7.2, and 6.8) on a 75-pm i. d. uncoated capillary, 60 cm long and 40 cm from anode to detector. Anolyte 10-mM phosphoric acid catholyte 20-mM NaOH. Running buffer contains 0.1% methylcellulose, 1% TEMED, and 5% Pharmalyte 3-10 carrier ampholytes. The UV 280-nm detection.11 [Reprinted, with permission, from J. R. Mazzeo and I. S. Krull, Anal. Chem. 63 (No. 24), 1991, 2852-2857. Capillary Isoelectric Focusing of Proteins in Uncoated Fused-Silica Capillaries Using Polymeric Additives . 1991 by American Chemical Society.]... Figure 12.13. CIEF of cytochrome c (peak 1, pi 9.6), chymotrypsinogen A (peak 2, pi 9.0) and myoglobin (peaks 3 and 4, pi 7.2, and 6.8) on a 75-pm i. d. uncoated capillary, 60 cm long and 40 cm from anode to detector. Anolyte 10-mM phosphoric acid catholyte 20-mM NaOH. Running buffer contains 0.1% methylcellulose, 1% TEMED, and 5% Pharmalyte 3-10 carrier ampholytes. The UV 280-nm detection.11 [Reprinted, with permission, from J. R. Mazzeo and I. S. Krull, Anal. Chem. 63 (No. 24), 1991, 2852-2857. Capillary Isoelectric Focusing of Proteins in Uncoated Fused-Silica Capillaries Using Polymeric Additives . 1991 by American Chemical Society.]...
See other pages where Capillary isoelectric focusing ampholyte buffers is mentioned: [Pg.176]    [Pg.68]    [Pg.110]    [Pg.671]    [Pg.684]    [Pg.476]    [Pg.14]    [Pg.176]    [Pg.76]    [Pg.190]    [Pg.40]    [Pg.156]    [Pg.88]    [Pg.273]   
See also in sourсe #XX -- [ Pg.671 ]



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Ampholyte

Ampholyte buffers

Ampholytes

Ampholytes buffer

Ampholytes isoelectric focusing

Ampholytic

Capillary isoelectric focusing

Isoelectric

Isoelectrical ampholyte

Isoelectrical focusing

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