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Calmodulin sensing system

Recently, a chameleon calcium reporter has been designed by R.Y. Tsien to sense calcium in cells by FRET. The structure of the system is based on a CEP separated from an YEP by the calmodulin calcium binding protein (CaM) and a calmodulin binding peptide (M13). If Ca ions are bound, CaM wraps around Ml 3 allowing high efficiency of excitation transfer from the donor CEP to the acceptor YEP (see Figure 9). The degree of FRET in chameleon is a sensitive ratiometric reporter (ratio 527/434 YPE/ CEP) of the calcium concentration in cells and in solution. The beauty of this approach is that the... [Pg.1393]

See other pages where Calmodulin sensing system is mentioned: [Pg.403]    [Pg.33]    [Pg.150]    [Pg.137]    [Pg.33]    [Pg.552]    [Pg.551]    [Pg.127]    [Pg.395]   
See also in sourсe #XX -- [ Pg.275 ]

See also in sourсe #XX -- [ Pg.275 ]



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