Caliper Labchip

Whereas fluorescence is typically measured during non-separation, mix-and-read protocols offering high readout throughput, several approaches exist today that involve separation steps in front of the actual detection. In the Caliper Labchip technology, a multi-parallel microfluidic separation system coupled with fluorescence detection allows the monitoring of enzymatic reactions. Quantification of substrate and product of the enzymatic reaction, after separation from each other as well as from the test compound, minimises artefacts and offers ratiometric results, although with comparatively lower... 

---