Calculations Involving Proteins Without Assignments

Selective saturation (using shaped pulses) of all the aromatic ring CH resonances together since they show up generally in a short window of 6.5-8.5 ppm. The Q-matrix is easily set up for this case. 

Similarly, the methyl resonances which appear as a group (generally between -1 ppm and 1.5 ppm) can be saturafed fogether and incorporafed in 

Finally, a saturation-transfer screening (using short saturation times) of the protein resonance envelope to identify irradiation frequencies that yield large STDs may be of some aid in the identification of residues within the binding pocket, and in assignments for the calculations. 

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