C Lipid Probes

Fluorescence polarization immunoassays (FPIs) are widely used to measure the amounts of drugs (D) and small molecides in chnical samples (Chapter 19). FPIs are based on the changes in polarizadon (or anisotropy) which occur when a labeled g analog (F-D) binds to an antibody specific filT that drag O igure 20.20). The anisotropy of the lafaded drag can be estiinated fiom the Perrin equation. [Pg.582]

Figim20.21. Buonuei pd riz lioaiiiiiiiunoiaiayof bigl -iiiolMlll - d(lllt Kck iiangaRii(Il)MljC. [Pg.583]

FPb of the high-iiiDfecular-wmgbt antigen HSA have been performed nang the Ru MLC probes. Ihese [Pg.583]

SMMoliapierfarpp)belifeliiBe fium4mlp4 is.Hiecorvr iiebisedonEq.(20Jl,aMniillllgVeA [Pg.583]

Tile hijdi quantum-yield ihenium MLC was covakndy bound to HSA and u to detect Umfing of an antibody against HSA Rugose 2035). The stead y-state anisotropy was found to increase nearly fourfold upon binding of IgG specific for HSA. and there was no effect from nonspecific [Pg.584]

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