C column the right choice for my sample

In view of the considerable number of C g columns available, it is unreasonable to test by trial and error many stationary phases for a new problem. If you do not have more detailed information, you should try to limit your choice. There are a few rules. 

The following solute characteristics are helpful for a first limitation  

For a start, you should decide whether your solute can in principle be separated on a C jj silica gel based stationary phase in a classical reversed-pha.se system. Subsequently, you can eliminate some columns based on solute characteristics and can focus on others. 

The sample is soluble in an acidic or alkaline solvent and most likely can be analyzed in it. 

The solutes possess a similar polarity, there are only minor structural differences. 

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