Butyryl dehydrogenase

Acyl CoA Dehydrogenases. Reaction (2) is catalyzed by a family of related enzymes. The lower fatty acid derivatives are oxidized by an enzyme purified from beef liver mitochondria. The isolated enzyme, butyryl dehydrogenase, has been reported to contain both riboflavin and copper.The nature of this enzyme will be discussed later in conjunction with other flavin enzjnmes. Chains of 3 to 8 carbons are oxidized in the presence of suitable electron acceptors, including 2,6-dichloro-phenolindophenol, cytochrome c, and ferricyanide. The maximum rate is obtained with butyryl CoA. It has been reported that the initial oxidation of fatty acid-CoA and the reduction of the employed electron acceptors are carried out by two different flavoproteins, which react with each... 

E. coli C. Kluyveri 4-hydroxy-butyryl-CoA dehydrogenase and R. eutropha phaC P(4HB) 4-hydroxybutyric acid and glucose 68... 

Methyl 3-hydroxy butyric aciduria 2-Methyl 3-hydroxy butyryl-CoA dehydrogenase... 

Butyryl-CoA dehydrogenase formation of long Steyn-Parve and Beinert 19)... 

For every step of the P oxidation sequence there is a small family of enzymes with differing chain length preferences.6 7 For example, in liver mitochondria one acyl-CoA dehydrogenase acts most rapidly on M-butyryl and other short-chain acyl-CoA a second prefers a substrate of medium chain length such as n-octanoyl-CoA a third prefers long-chain substrates such as pal-mitoyl-CoA and a fourth, substrates with 2-methyl branches. A fifth enzyme acts specifically on isovaleryl-CoA. Similar preferences exist for the other enzymes of the P oxidation pathway. In Escherichia coli... 

In applying mutasynthesis to the production of novel avermectins, the elimination of branched-chain a-keto acid dehydrogenase (BCDH) was targeted. This multienzyme complex is responsible for supplying the 2-methylbutyryl- and iso-butyryl-CoA starter units that initiate natural avermectin biosynthesis [21,29],... 

Fendrich G, Abeles RH. Mechanism of action of butyryl-CoA dehydrogenase-reactions with acetylenic, olefinic, and fluorinated substrate-analogs. Biochemistry 1982 21 6685-6695. 

Matern D, He M, Berry SA, Rinaldo P, Whitley CB, Madsen PP, et al. Prospective diagnosis of 2-methyl-butyryl-CoA dehydrogenase deficiency in the Hmong population by newborn screening using tandem mass spectrometry. Pediatrics 2003 112 74-8. 

Butyryl CoA Dehydrogenase Bovine liver mitochondria Cu 3,450 Mahler (1954)... 

Acyl-CoA dehydrogenase (especially butyryl-CoA dehydrogenase) is irreversibly inactivated by methylene cyclopropylacetyl-CoA through the formation of covalent adduct with the FAD of the enzyme. The inhibitor is derived by transamination... 

Fig. 12. Mechanism proposed for inactivation of butyryl-CoA dehydrogenase by (3-cbloro-3-butenoyl)pantetheine.

The ( H. H)acetoacetyl-CoA species were synthesized from (3S)-3-hydroxy(2- H2Jbutyryl-CoA and (3S)-3-hydroxy[2- H2]butyryl-CoA by the sequential use of enoyl-CoA hydratase (in HjO or HOH, respectively) and (3S)-specific 3-hydroxyacyl-CoA dehydrogenase (211). 

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