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Buffered weak detergent solution

Respiring mitochondria extrude protons. A weakly buffered suspension of mitochondria is provided with an oxidizable substrate and allowed to use up all the O2 in the solution. The traces show measurements of the pH of the suspension, with pH decreases plotted upward. When a small amount of O2 is added (upward-pointing arrow), respiration can occur for a few seconds. The pH of the solution decreases suddenly at this point (Exp. A). The pH change persists for a period of several minutes, long after the 02 has been used up. If an uncoupler is added, the pH returns abruptly to nearly its original level. Experiment B was done in the presence of a detergent that disrupted the inner membrane, making the membrane... [Pg.320]

See other pages where Buffered weak detergent solution is mentioned: [Pg.83]    [Pg.83]    [Pg.108]    [Pg.98]    [Pg.64]    [Pg.104]    [Pg.448]    [Pg.512]    [Pg.275]    [Pg.17]    [Pg.621]    [Pg.213]    [Pg.267]   
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Buffered solution

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