BSA assay

For BSA assay, to 1.5 ml of buffer-BSA solution, typically 40 fi of a 1 100 dilution of rabbit anti-BSA (2.4 mg/ml) and 40 /aI of a 1 10 dilution of urease-BSA conjugate were added. For cAMP assay, 30 ju.1 of a 1 10 dilution of the (NH4)2S04 fraction of cAMP antiserum (0.5 mg/ml) and 30/al of a 1 10 dilution of enzyme-cyclic nucleotide conjugate were added to 1.0 ml of buffer or buffer-nucleotide standard. 

---