Bovine serum albumin, spectra samples

FIGURE 24 Positive ion ESP mass spectrum of bovine serum albumin. The average molecular mass observed here for this protein is 66,424 Da. There are two additional higher mass proteins in this sample. 

Figure 4.9 IR spectrum of deuterated Bovine Serum Albumine (BSA) at room temperature (upper spectrum). Same spectrum minus spectrum of the same sample at 116 °C (lower spectrum). Spectra are offset for clarity.

The Bradford method is based on the absorbance of Coomassie Brilliant Blue on the protein to be determined, which results in a spectrum shift of the dye from 465 to 595 nm. The absorbance at 595 nm is proportional to the protein concentration. Bovine serum albumin is used as standard. The reaction is susceptible to interferences from surfactants and alkalinity of the solution. The main advantage of the method is rapidity, which enables efficient application to many samples. 

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