Bovine pancreatic chymotrypsinogen

Chymotrypsin has also been utilized to promote debridement, as well as the reduction of soft tissue inflammation. It is also used in some opthalmic procedures, particularly in facilitating cataract extraction. It is prepared by activation of its zymogen, chymotrypsinogen, which is extracted from bovine pancreatic tissue. 

Fig. 1. Chromatography of bovine pancreatic juice on DEAE-cellulose (anionic proteins) and Amberlite IRC-50 (cationic proteins) (1). RNAase, ribonuclease ChTg-a, chymotrypsinogen A Tg, trypsinogen ProCp-B and Cp-B, procarboxypeptidase B and carboxypeptidase B DNAase, deoxyribonuclease ProCp-A, procarboxypeptidase A.

The second, anionic bovine chymotrypsinogen (chymotrypsinogen B), crystallized by Laskowski et al. (46), was initially thought to be of minor importance. This impression probably arose because of heavy losses during purification. Direct chromatography (1) proved later that the amounts of chymotrypsinogens A and B in bovine pancreatic juice were in fact very similar. Therefore it became important to compare as closely as possible the structures and modes of activation of two chymotrypsinogens synthesized by the same species. 

Results of protein-protein docking. aCHYN-a-chymotrypsinogen, a-CHY-a-chymotrypsin, HPTI-human pancreatic trypsin inhibitor, BPTI-bovine pancreatic trypsin inhibitor, CHYI-chymotrypsin inhibitor, Subtilisin l-subtilisin inhibitor D1.3, D44.1, HyHEL5 and HyHELlOare monoclonal antibodies. For further details of coordinates see Cabb et al. [12]. In the table some degenerate identical complexes included our earlier studies have been excluded. 

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