Bovine insulin absorption

When diabetic rabbits (24) were treated with 50 IU of bovine insulin imbibed at 50 mg/g poly (acrylic acid) (Figure 14) no reduction in serum glucose over that achieved by the dry blend control could be detected. Pretreatment of the animals with oral doses of either a penetration enhancer, sodium taurocholate, or a protease inhibitor, aproteinin, failed to improve the insulin activity. One possible explanation for this unexpected lack of activity might be that the diseased animals exhibit impaired ileal absorption of fluids (25). 

FIGURE 13.17 Absorption (top) and emission spectra (bottom) of PTAA (O), PTAA-native bovine insulin ( ) and PTAA-amyloid fibrillar bovine insulin (x). 

Figure 22.8 Absorption spectra of (a) 80 nM of polymer 11 (on a monomer basis) in 20 mM sodium phosphate, pH 7.0, (b) in 20 mM sodium phosphate pH 7.0 with 5.0 icM of native bovine insulin (nBI) and (c) in 20 mM sodium phosphate, pH 7.0, with 5.0 fx.M of amyloid fibrils of bovine insulin (fBI). The inset shows the microtiter plate wells containing polymer 3-nBI (left) and polymer 3 fBI (right). Reprinted with permission from K. P. R. Nilsson, A. Herland, P. Hammarstrom and O. Inganas, Conjugated poly electrolytes conformation-sensitive optical probes for detection of amyloid fibril formation. Biochemistry, 44, 3718-3724 (2005). Copyright 2005 American Chemical Society...

Hddebrandt, P., Birch, K., Sestoft, L., and Volund, A., 1984a, Dose-dependent subcutaneous absorption of porcine, bovine and human NPH insulins, Acta Med. Scand 215 69-73. 

Hildebrandt, P., Berger, A., Volund, A., and Kiilil, C., 1985a, The subcutaneous absorption of human and bovine ultralente insulin formulations. Diabetic Med 2 355-359. 

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