Boron dipyrromethane BODIPY

Membrane-bound probes, which are incorporated directly during vesicle preparation, are commonly fluorescently labeled phospholipids. These probes are mainly used to address more complex changes in membrane structure, thereby providing insights into the structure and mechanism of the transporters. Depending on the specific desired application, various fluorescently labeled phospholipids are available. Popular examples include boron dipyrromethane (BODIPY) probes as FRET acceptors, DOXYL probes as quenchers for parallax analysis, 7-nitro-benzofurazan (NBD) probes such as NBD-POPE for flip-flop assay, and so on (Figure 8). 

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