Bond orders, methyl thiazoles

Boimd to 1, the intercalated bithiazole is positioned with its C-terminal thiazole ring sandwiched between Aio and An while the N-terminal thiazole occupies a more central position between the base pairs involving these same residues the helix is unwound by 8.9. In this position, the guanidinium tail is threaded through to the major groove and forms a water-mediated H-bond to the N7 of Aio. Connected to the bithiazole, the Thr-methylvalerate linker is well ordered and makes two specific H-bonds to the sugar-phosphate backbone these interactions include the methyl valerate hydroxyl group to the 03 of T6 and the bithiazole NH (amide link to Thr) to the 04 of T7. 

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