Blood coagulation proteins, electrophoresis

We have reported that bulbs of Crocus sativus contain a poly-histidine protein factor, which aggregates human platelets in vitro. The molecular weight of this factor as found by gel filtration chromatography on a Sephadex G-75 column and sodium dodecylsulfate (SDS) polyacrylamide slab gel electrophoresis is 42,000Da [79], In addition, another low molecular weight protein factor isolated from the same crude extract was found to inhibit human platelet aggregation in vitro [80]. The effect of Crocus sativus on blood coagulation has been also examined by Nishio et al [81]. 

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