Biotin terminated

Chen B, Metera K, Sleiman HF. Biotin-terminated ruthenium hipyridine ring-opening metathesis polymerization copolymers synthesis and self-assemhly with streptavidin. Macromolecules 2005 38 1084-1090. 

Synthesis of phorboxazole A has been accomplished [8, 9], The C(46) terminus of phorboxazole A was modified to incorporate a biotin-terminated linker via a direct Sonogashira reaction with tris-polyethyleneglycol vinyl iodide-biotin ester using catalytic PdCljlPPh, ), Cul, and NEt, in THE This process demonstrated the utility of mild C-C bond formation in the context of the phorboxazole architecture and provided a potential affinity probe [10],... 

Fig. 7 Frequency responses of streptavidin-treated QCMs (10 MFIz) to (1) probe (biotin-terminated 30-mer nucleotide) immobilization, (2) MMO target (upper curve) and MMl target (lower curve) DNA hybridization, and (5) MutS protein binding. Arrows (3), (4), and (6) show the change in buffer system. (From Su et al. [53].)...

Fig. 23 A Chemical structures of biotin-terminated LKL16 and PNIPAM used as building units for the thermo-responsive nanofiber architecture. B Schematic illustration of the nano-organization of PNIPAM onto biotin-LKL16/LKL16-mixed nanofiber template through biotin-SAv complexation. C TEM images of PNIPAM/SAv-immolized nanofibers prepared onto carbon-coated copper grids at 20°C (below LCST of PNIPAM) or 45 °C (above LCST of PNIPAM)...

Figure 11.8 Biotin-BMCC provides sulfhydryl reactivity through its terminal maleimide group. The reaction creates a stable thioether linkage.

Figure 17.3 Maleimide-modified glass slides (1) can be derivatized using two chemoselective ligation reactions to create biotin modifications. In the first step, alkyne-PEG4-cyclopentadiene linkers (2) are added to the maleimide groups using a Diels-Alder reaction. In the second reaction, an azido-PEG4-biotin compound (3) is reacted with the terminal alkyne on the slide using click chemistry to result in another cycloaddition product, a triazole ring.

Once the protein is modified to contain an alkynyl group at its C-terminal it can be used to covalently link to its click chemistry reactant partner, an azide on the surface of an array. Other azido molecules also can be conjugated with an alkyne-protein to facilitate the detection or capture of the protein using affinity techniques. For instance, an azido-fluorescein reagent can be used to detect fluorescently the expressed protein in complex samples or an azido-biotin... 

Figure 17.21 An azido-farnesyl diphosphate derivative can be added to cells to obtain farnesylated proteins containing terminal azide groups that can be targeted in a Staudinger ligation reaction. Biotinylation of these post-translationally modified proteins can be done in vivo using a biotin-phosphine derivative.

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