Biotin, radioiodination

Wilbur, S.D., Pathare, P.M., Hamlin, D.K., Buhler, K.R., and Vessella, R.L. (1998) Biotin reagents for antibody pretargeting. 3. Synthesis, radioiodination, and evaluation of biotinylated starburst dendrim-ers. Bioconjugate Chem. 9, 813-825. 

They have been used to label a wide variety of molecules, e.g., hormones (Massaglia et ai, 1973) or biotin (Foulon et ai, 1996). The presence of the active aromatic group enables the use of milder radioiodination conditions. Vinylic prosthetic groups have been developed to minimize structural perturbations on the target molecule. This is also the case of the beta-iodoethoxyl group (Hamant et ai, 1994) used to label a very small molecule hexanoic acid. 

Wilbur, D. S., Hamlin, D. K., Chyan, M.-K. et al. 2004b. Biotin reagents in antibody pretargeting. 6. Synthesis and in vivo evaluation of astatinated and radioiodinated aryl- and wido-carboranyl-biotin derivatives. Bioconjugate Chem., 15, 601-16. 

Figure 21. Radioiodinated boron-containing derivatives of biotin.

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