Biotin/avidin. protein immobilization

To efficiently monitor the interaction between a small molecule and a protein expressed on the phage surface, the small molecule is to be immobilized on the solid surface. Generally, biotin-avidin interaction has been used for this purpose. Biotin is covalently linked to the specific functional group of a small molecule that does not affect its biological effect on cells. Small molecules can be linked... 

Biotin/avidin chemistry has been used extensively for the immobilization of proteins at electrode surfaces [2]. The interachon between one molecule of... 

Patterning of enzyme monolayers on a solid surface was carried out by photoactivation of immobilized monolayer of caged -biotin derivatives in selected areas. Specific oriented binding of enzyme-avidin conjugates could be readily made to the photoactivated zones [42]. Oriented immobilization of G-protein-coupled receptors on a solid surface was also made possible on a biotinylated surface by first immobilizing streptavidin, followed by the immobilization of biotinylated G-protein-coupled receptor [43]. 

The reagent also has been used in a unique tRNA-mediated method of labeling proteins with biotin for nonradioactive detection of cell-free translation products (Kurzchalia et al., 1988), in creating one- and two-step noncompetitive avidin-biotin immunoassays (Vilja, 1991), for immobilizing streptavidin onto solid surfaces using biotinylated carriers with subsequent use in a protein avidin-biotin capture system (Suter and Butler, 1986), and for the detection of DNA on nitrocellulose blots (Leary et al., 1983). 

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