Biosensors sensitivity enhancement strategies

To complete this overview on the strategies that are pursued to enhance the sensitivity and stability of screen-printed biosensors for the detection of pesticides new immobilization approaches have to be mentioned. SPE modified with concanavalin A have been used to bind, via a high affinity interaction, AChE. The obtained biosensors, optimized for manufacturing conditions, were able to detect 10 8 M of clorpyrifos [45]. 

In our laboratory, D1 protein of algae and plants vras chosen as a biomediator and its overexpression in heterologous hosts was a mandatory step to constmct new biosensor prototypes. This manuscript focuses on a few strategies usefiil to produce D1 protein based sensors. Subsequently, we describe some bioinformatic approaches to establish amino acid mutations, which can specifically enhance sensitivity and selectivity of sensing elements towards pollutants. 

In conjunction with appropriate measuring regimes not only does a much wider range of analyte species becomes in this way accessible to measurement by the bioelectroanalytical approach also the selectivity and sensitivity of the biosensor may be enhanced through the appropriate choice of the coupling strategy [2,14],... 

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