Biosensors binding

Schuck P 1996 Kinetics of iigand binding to receptors immobiiized in a poiymer matrix, as detected with an evanescent wave biosensor, i. A computer simuiation of the influence of mass transport Biophys. J. 70 1230-49... 

The low detection limit, high sensitivity, and fast response times of chemoreceptor-based biosensors result primarily from the extremely high binding constants of the receptor R for the target substrate S. The receptor—substrate binding may be described... 

The immobilization of the hyperbranched spherical structures onto physical transducers greatly increases the binding capacity of the surface and leads to enhanced sensitivity and extended linearity of biosensors. Nucleic acid dendrimers were prepared and their amplification properties for the detection of DNA were examined using mass-sensitive transducers [45, 46]. Antibodies... 

It is critical to keep in mind that existing reagents can be used for multiple formats. Eor example, polyclonal antibodies dominate the environmental field because they generally provide greater sensitivity and specificity for small molecules at a much lower cost than do monoclonal or recombinant antibodies. With some biosensors monoclonal or engineered antibodies or recombinant binding proteins may offer advantages. 

The initial hurdle to overcome in the biosensor application of a nucleic acid is that involving its stable attachment on a transducing element which commonly includes a metallic electrode. In the first part of this chapter, we wish to introduce our approach for DNA immobilization (Scheme 1). A detailed characterization of the immobilization chemistry is also presented. In the second part, we follow the development of work from our laboratory on chemical sensor applications of the DNA-modified electrode involving a biosensor for DNA-binding molecules and an electrochemical gene sensor. 

Figure 7.9. Schematic diagram of a surface plasmon resonance biosensor. One of the binding partners is immobilized on the sensor surface. With the BIACORE instrument, the soluble molecule is allowed to flow over the immobilized molecule. Binding of the soluble molecule results in a change in the refractive index of the solvent near the surface of the sensor chip. The magnitude of the shift in refractive index is related quantitatively to the amount of the soluble molecule that is bound.

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