Biomolecular Quenching Constants

In this equation, F0 and F are the fluorescence intensities in the absence and presence of the quencher, t0 and x are the lifetimes of the fluorophore in the absence and presence of the quencher, kq is the biomolecular quenching constant, and [Q] is the concentration of the quencher (in this case, molecular oxygen). The Stem-Volmer quenching constant is Kn, and is calculated as the product of kq and r0. The Smoluchowski equation describes the biomolecular quenching constant, kq, and is given by... 

TABLE 2.2 Biomolecular Quenching Constants Derived from Various Labeled PMAA Samples... 

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