Biomarker Identification and Validation

Most common techniques available for biomarker discovery, characterization, separation, and identification 

Good for proteins, ease of use, good analysis tools, rapid, very robust, sample can be recovered High resolution, well-established method, good analysis tools, sample can be recovered 

High resolution, small sample size, rapid, not affected by size of the analyte, reproducible High resolution, possible to combine column types to produce 2D separations, not affected by size of the analyte, wide range of separation methods available, very robust, reproducible, sample can be recovered, can be coupled to a wide variety of detector types 

Very high resolution, can be coupled to mass spectrometry for detection and identification, robust, good for small volatile molecules, reproducible, very small sample size, rapid Rapid, reproducible, 2D separations standard, wide range of separation types available, robust, small sample size 

Very sensitive, large number of stains available, robust, simple, inexpensive, permits sample recovery, very widely used 

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