Biological Material barbiturates

B27. Broughton, P. M. G., A rapid ultraviolet spectrophotometric method for the detection, estimation and identification of barbiturates in biological material. Biochem. J. 63, 207-215 (1956). 

One of the problems of barbiturate analysis is the isolation of the compound from biological material.427,428,437,449,513 Extraction is the most accepted method, but some assays take advantage of omitting the isolation and purification step. Special attention was devoted to separation and assay of particular barbiturate drugs in combination with other compounds. For example, many methods were devised for the determination of phenobarbital and hydantoin used together in anticonvulsive therapy.514 Several procedures were proposed for the determination of barbiturates along with their metabolites in body fluids.437... 

Determination of Barbiturates in Biological Material with Chrom 3, a Czechslovak Chromatograph Soudni Lek. 20(3) 38-41 (1975) CA 85 71948f... 

Adsorption of biologically important materials, such as fibrinogen from plasma proteins and barbiturates, " and organic molecules including corrosion inhibitors have been studied, often as functions of potential of the substrate, by ellipsometry in situ. Jovancicevic, Yang, and Bockris used time-resolved ellipsometry to measure the thickness, refractive index, and extinction coefficient of the adsorption layer of l-octyne-3-ol on iron as functions of time. They showed that the layer thickness increased stepwise, due to change of the adsorption configuration from flat... 

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